We have simplified few molecular techniques and instruments that can ease infection detection and scores of other research activities economically. These include two homemade instruments, two homemade inexpensive and stable field Surveillance/ detection kits and few improved methods. These, will have multifarious applications even in remote areas. The two field “DNA Surveillance/ Diagnostic Kits” are expected to have infinite shelf life at room temperature (we have tested its feasibility up to three years) and it is expected to be the least expensive in the world. The well-known standard protocols like DNA extraction, PCR, hybridization etc., unless improved by us, are not reported here but need to be followed wherever required. These instruments, methods and concepts can be used under field conditions by semi-skilled workers.
Author(s) Details:
Lahiri S. S.,
Defence R & D Est., D.R.D.O., Gwalior, Madhya Pradesh, India and Institute of Nuclear Medicine and Allied Science (INMAS), DRDO, New Delhi, India and Amity University, Noida, Uttar Pradesh, India and Amity University, Noida, Uttar Pradesh, India.
Singh R.,
Defence R & D Est., D.R.D.O., Gwalior, Madhya Pradesh, India.
Karothia B. S.,
Defence R & D Est., D.R.D.O., Gwalior, Madhya Pradesh, India.
Giredhar M.,
Center for Bio-Hybrid Medical Systems, Forckenbeckstr. 55, 52074 Aachen, Germany.
Chakraborty A.,
Division of Immunology, Immunity to Infection and Respiratory Medicine, School of Biological Sciences, The University of Manchester, Manchester, M13 9PT, UK.
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