This chapter focuses on the production of lipase by a biosurfactant producing Acinetobacter sp. YMP. This species demonstrated potential for vegetable oil degradation on Mineral Salt Medium [MSM], and was thus studied for its lipase production. The production of lipase was confirmed on fat agar, and its activity was measured by the titrimetric method. The Acinetobacter species YMP was isolated from oily waste collected from Farsan industry located at Unchgaon, Kolhapur. The study also investigated the impact of various environmental factors such as agitation, time span, substrate concentration, pH, and temperature on lipase production using Acinetobacter sp. YMP. The enzyme was purified using the ammonium sulphate (60%) precipitation technique. The maximum lipase production was found to be achieved within 120 hours at 450 rpm, 3.0 pH, 27°C temperature, and at 2% substrate concentration. The species also demonstrated an oil degradation potential of up to 8% on MSM broth and agar. Overall, the study highlights the potential of Acinetobacter sp. YMP for lipase production and its ability to degrade vegetable oil.
Author(s) Details:
Mrudula Madhusudan Bendigeri,
Department of Food Science and Technology, Shivaji University, Kolhapur, 416004, Maharashtra, India.
Yasmin Chand Attar,
Department of Food Science and Technology, Shivaji University, Kolhapur, 416004, Maharashtra, India.
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